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Elisa Kits 2
Prominent & Leading Manufacturer from Ahmedabad, we offer drg dihydrotestosterone (dht) elisa kit, calbiotech folate elisa kit, calbiotech ca 15-3 elisa kit, calbiotech ca 19-9 elisa kit, anti ccp elisa kit and calbiotech androstenedione elisa kit.
DRG Dihydrotestosterone (DHT) Elisa Kit
Product Price: Rs 16,000 / KitGet Best Price
Minimum Order Quantity: 1 Kit
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Product Details:
| Category | Elisa |
| Product type | Elisa Kit |
| Usage/Application | Hospital / Pathlab |
| Packing Type | Box |
| Pack Size | 96 Wells |
The principle of the following enzyme immunoassay test follows the typical competitive binding scenario. Competition occurs between an unlabeled antigen (present in standards, control and specimen samples) and an enzyme-labelled antigen (conjugate) for a limited number of antibody binding sites on the microwell plate. The washing and decanting procedures remove unbound materials. After the washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the colour formed is inversely proportional to the concentration of DHT in the sample. A set of standards is used to plot a standard curve from which the amount of DHT in samples and controls can be directly read.
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- Delivery Time: Immediate
- Packaging Details: 1 Kit
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Calbiotech Folate Elisa Kit
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Product Details:
| Number of Reactions(Preps)/Kit | 96 Wells |
| Brand | Calbiotech |
| Product Type | ELISA Kit |
| Packaging Type | Box |
| Usage/Application | Hospital / Pathlab |
| Category | Elisa |
The Folate test kit is a solid phase enzyme-linked immunoassay (ELISA), based on the principal of competitive binding. Streptavidin coated wells are co-incubated with extracted Folate standards, controls, samples, Folate Binding Protein-Biotin conjugate and Folate-Enzyme conjugate at room temperature for 60 minutes. During the incubation, the folate-enzyme conjugate and the folate in the sample, standard, or quality control serum will compete to bind to the biotin-labeled folate binding protein. After the 60 minute incubation, all unbound conjugates are then removed and the wells are washed. Next, a solution of TMB Reagent is added and incubated at room temperature for 15 minutes, resulting in the development of blue color. The color development is stopped with the addition of stop solution, and the absorbance is measured spectrophotometrically at 450 nm. A standard curve is obtained by plotting the concentration of the standard versus the absorbance. The color intensity is inversely proportional to the amount of Folate in the sample. The total assay procedure run time is 1.25 hours.
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- Delivery Time: Immediate
- Packaging Details: 1 Kit
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Calbiotech CA 15-3 Elisa Kit
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Product Details:
| Minimum Order Quantity | 1 Kit |
| Brand | Calbiotech |
| Number of Reactions(Preps)/Kit | 96 Wells |
| Product Type | ELISA Kit |
| Packaging Type | Box |
| Usage/Application | Hospital / Pathlab |
| Storage and Stability | Store the kit at 2-8 Degree C |
The CA15-3 ELISA test is an adapted solid phase sequential sandwich ELISA. Samples and biotinylated monoclonal antibody are added to wells coated with streptavidin. CA15-3 in the patient sample binds to biotinylated capture antibody. The biotinylated antibody simultaneously binds to the streptavidin coated plate. After a wash step, anti-CA15-3–HRP enzyme conjugate is added and forms a sandwich around captured CA15-3. Unbound antibodies are washed off. TMB substrate is added resulting in the development of a blue color. The concentration of CA15-3 is directly proportional to the color intensity developed. A standard curve is generated relating color intensity to CA15-3 concentration.
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- Delivery Time: Immediate
- Packaging Details: 1 Kit
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Calbiotech CA 19-9 Elisa Kit
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Product Details:
| Minimum Order Quantity | 1 Kit |
| Pack Size | 96 Wells |
| Product type | Elisa |
| Storage and Stability | Store the kit at 2 - 8 Degree |
| Brand | Calbiotech |
| Species | Human |
| Packaging Type | Box |
Principle of the test the CA 19-9 elisa test is an adapted solid phase sequential sandwich elisa. Samples and biotinylated monoclonal antibody are added to wells coated with streptavidin. CA 19-9 in the patient sample binds to biotinylated capture antibody. The biotinylated antibody simultaneously binds to the streptavidin coated plate. After a wash step, anti-CA 19-9–hrp enzyme conjugate is added and forms a sandwich around captured CA 19-9. Unbound antibodies are washed off. Tmb substrate is added resulting in the development of a blue color. The concentration of CA 19-9 is directly proportional to the color intensity developed. A standard curve is generated relating color intensity to CA 19-9 concentration.
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- Delivery Time: Immediate
- Packaging Details: 1 Kit
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Anti CCP Elisa Kit
Product Price: Rs 29,500 / KitGet Best Price
Minimum Order Quantity: 1 Kit
Product Details:
| Number of Reactions(Preps)/Kit | 96 Wells |
| Brand | DRG |
| Packaging Type | Box |
| Method | Elisa |
| Bio-Type | Serum, Plasma |
| Usage/Application | Hospital / Pathlab |
- The microwells are coated with true CCP antigens specific for Rheumatoid arthiritis diagnosis and therapy monitoring.
- Quantitative determination.
- Standard range is 0-1600 AU/ml.
- 2 level controls available
- Breakable microwells & ready to use reagents.
- Colour coded reagents
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- Delivery Time: Immediate
- Packaging Details: 1 Kit
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Calbiotech Androstenedione Elisa Kit
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Product Details:
| Minimum Order Quantity | 1 Kit |
| Number of Reactions(Preps)/Kit | 96 Wells |
| Brand | Calbiotech |
| Packaging Type | Box |
| Usage/Application | Hospital / Pathlab |
| Species | Human |
| Category | Elisa Kit |
Androstenedione is the primary precursor of testosterone in women. It is synthesized in the adrenal gland. Measurement of Androstenedione may be used as an indicator of androgenic activity in women. The steroid hormone Androstenedione is one of the main androgens, besides Testosterone and Dehydroepiandrosterone. In males, androgens are secreted primarily by the Leydig cells of the testes, to some degree also in the adrenal cortex. In females, the androgens are secreted mainly in the adrenal glands and in the ovary. Around 10% of the androgens are derived from peripheral conversion, mainly of DHEA. Androstenedione and Testosterone show high diurnal variability. The highest levels are measured in the morning. At the age of puberty serum androstenedione levels rise, after menopause they decline again. High androstenedione levels are measured during pregnancy. In women, high levels of androstenedione (47-100% above normal) are generally found in hirsutism, mostly in combination with other androgens as testosterone and DHEA-S. Androstenedione overproduction is due to ovarian dysfunction or maybe of adrenal origin. High circulating androstenedione levels are found in women with polycystic ovaries and 21-hydroxylase effect. Significant lower androstenedione levels are found in postmenopausal osteoporosis.
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- Packaging Details: 1 Kit
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Calbiotech FT4 Elisa Kit - Thryoxine
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Product Details:
| Minimum Order Quantity | 1 Kit |
| Number of Reactions(Preps)/Kit | 96 Wells |
| Brand | Calbiotech |
| Product Type | ELISA Kit |
| Packaging Type | Box |
| Species | Human |
| Usage/Application | Hospital / Pathlab |
The CBI fT4 is a solid phase competitive ELISA. The samples Anti-T4 Biotin and fT4 enzyme conjugate are added to the wells coated with Streptavidin. fT4 in the patient’s serum competes with a T4 enzyme conjugate for binding sites. Unbound T4 and T4 enzyme conjugate is washed off by washing buffer. Upon the addition of the substrate, the intensity of color is inversely proportional to the concentration of fT4 in the samples. A standard curve is prepared relating color intensity to the concentration of the fT4.
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- Delivery Time: Immediate
- Packaging Details: 1 Kit
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Calbiotech DHEA-S Elisa Kit
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Product Details:
| Number of Reactions(Preps)/Kit | 96 Wells |
| Brand | Calbiotech |
| Product Type | ELISA |
| Packaging Type | Box |
| Usage/Application | Hospital / Pathlab |
| Species | Human |
The CBI DHEA-S kit is based on the principle of competitive binding between DHAEA-S in the test specimen and HRP conjugated DHEA-S for a constant amount of anti-DHEA-S antibody. In the first incubation, Goat-anti-Rabbit-IgG coated wells are incubated with 10μl of DHEA-S standards, patient samples, 50μl DHEA-S Enzyme (HRP) reagent and 50μl anti-DHEA-S Antibody reagent, at room temperature, for 60 minutes. During the incubation, HRP labeled DHEA-S competes with the endogenous DHEA-S in the standard and sample, for a fixed number of binding sites of the DHEA-S antibody, while simultaneously the Anti DHEA-S antibody binds to the immobilized secondary antibody. Thus, the amount of DHEA-S HRP conjugate immunologically bound to the well progressively decreases as the concentration of DHEA-S in the specimen increases. Unbound DHEA-S HRP conjugate is then removed and the wells washed. Next, TMB Reagent is added and incubated at room temperature for 30 minutes, resulting in the development of blue color. The color development is stopped with the addition of stop solution, and the absorbance is spectrophotometrically measured at 450nm. A standard curve is prepared relating color intensity to the concentration of DHEA-S.
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- Delivery Time: Immediate
- Packaging Details: 1 Kit
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Calbiotech Estriol, Free Elisa Kit
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Product Details:
| Number of Reactions(Preps)/Kit | 96 Wells |
| Brand | Calbiotech |
| Product Type | ELISA |
| Packaging Type | Box |
| Usage/Application | Hospital / Pathlab |
| Species | Human |
Diluted patient serum is added to wells coated with purified antigen. IgG specific antibody, if present, binds to the antigen. All unbound materials are washed away and the enzyme conjugate is added to bind to the antibody-antigen complex, if present. Excess enzyme conjugate is washed off and substrate is added. The plate is incubated to allow the hydrolysis of the substrate by the enzyme. The intensity of the color generated is proportional to the amount of IgG specific antibody in the sample
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- Packaging Details: 1 Kit
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Calbiotech Estrogens, Total Elisa Kit
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Product Details:
| Number of Reactions(Preps)/Kit | 96 Wells |
| Brand | Calbiotech |
| Product Type | ELISA |
| Packaging Type | Box |
| Usage/Application | Hospital / Pathlab |
The Calbiotech Inc., Total Estrogens ELISA kit is based on the principle of competitive binding between Estrogens in the test specimen and enzyme labeled estrogens for a constant amount of rabbit antiestrogens antibody. In the first incubation, goat anti-rabbit IgG-coated wells are incubated with 50µl of Total Estrogens standards, controls, patient samples, 100µl Estrogens-HRP conjugate reagent and 50µl rabbit anti-Total Estrogens reagent, at room temperature, for 120 minutes, with agitation. During the incubation, HRP labeled Total Estrogens competes with the endogenous estrogens in the standard, controls and sample, for a fixed number of binding sites of the specific anti Total Estrogens antibody. Thus, the amount of HRP labeled Estrogens conjugate immunologically bound to the antibody progressively decreases as the concentration of Total Estrogens in the specimen increases. Unbound Total estrogens enzyme conjugate is then removed and the wells washed. Then substrate (TMB) reagent is added and incubated, with agitation, for 30 minutes, resulting in the development of blue color. The color development is stopped with the addition of 50µL stop solution, and the absorbance is spectrophotometrically measured at 450nm. A standard curve is prepared relating color intensity to the concentration of Total Estrogens versus the absorbance.
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- Delivery Time: Immediate
- Packaging Details: 1 Kit
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Calbiotech Progesterone Elisa Kit
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Product Details:
| Number of Reactions(Preps)/Kit | 96 Wells |
| Brand | Calbiotech |
| Product Type | ELISA |
| Packaging Type | Box |
| Usage/Application | Hospital / Pathlab |
| Species | Human |
The Calbiotech, Inc progesterone is a solid phase competitive ELISA. The samples, working progesteroneenzyme (HRP) Conjugate and anti-progesterone-Biotin reagent are added to the wells coated with Streptavidin. Progesterone in the patient’s specimen competes with a progesterone HRP conjugate for binding sites. Unbound Progesterone and Progesterone enzyme conjugate is washed off by washing buffer. Upon the addition of the TMB substrate, the intensity of color is inversely proportional to the concentration of Progesterone in the samples. A standard curve is prepared relating color intensity to the concentration of the Progesterone
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- Packaging Details: 1 Kit
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Calbiotech 17(OH) ProgesteroneElisa Kit
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Product Details:
| Number of Reactions(Preps)/Kit | 96 Wells |
| Brand | Calbiotech |
| Product Type | ELISA |
| Packaging Type | Box |
| Category | Elisa |
| Usage/Application | Hospital / Pathlab |
The Calbiotech 17α-OH Progesterone (17-OHP) is a based on the principle of competitive binding between 17- OHP in the test specimen and 17-OHP-HRP for a constant amount of Rabbit anti-17-OHP antibody. In the assay, goat anti-rabbit IgG-coated wells are incubated with 25µl 17-OHP standards, 50µl 17-OHP-HRP conjugate, and 50µl rabbit anti-17-OHP. HRP-labeled-17-OHP competes with 17-OHP in the standards and sample for a fixed number of binding sites of the specific anti-17-OHP antibody. Thus, the amount of 17-OHP peroxidase conjugate immunologically bound to the well progressively decreases as the concentration of 17- OHP in the specimen increases. Unbound 17-OHP is then removed by the washing steps. TMB Substrate is added, resulting in the development of blue color. The color development is stopped with the addition of stop solution, and the absorbance is spectrophotometrically measured at 450nm. A standard curve is prepared relating color intensity to the concentration of 17-OHP.
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- Delivery Time: Immediate
- Packaging Details: 1 Kit
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Calbiotech Testosterone, Free Elisa Kit
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Product Details:
| Number of Reactions(Preps)/Kit | 96 Wells |
| Brand | Calbiotech |
| Product Type | ELISA |
| Packaging Type | Box |
| Usage/Application | Hospital / Pathlab |
| Species | Human |
| Category | Elisa |
The CBI Testosterone ELISA kit is based on the principle of competitive binding between testosterone in the test specimen and Testosterone-HRP conjugate for a constant amount of rabbit anti-Free Testosterone. In the incubation, goat anti-rabbit IgG-coated wells are incubated with 25μl of Testosterone standards, patient samples, 50μl testosterone-HRP conjugate reagent and 50μl rabbit anti-free testosterone reagent at room temperature for 60 minutes. During the incubation, a fixed amount of HRP labeled testosterone competes with the endogenous testosterone in the standard and sample, for a fixed number of binding sites of the specific free testosterone antibody. Thus, the amount of testosterone peroxidase conjugate immunologically bound to the well progressively decreases as the concentration of free testosterone in the specimen increases. Unbound testosterone peroxidase conjugate is then removed and the wells washed. Next, 100µl of TMB Reagent is added and incubated at room temperature for 15 minutes, resulting in the development of blue color. The color development is stopped with the addition of 50µl stop solution, and the absorbance is measured spectrophotometrically at 450nm. A standard curve is prepared relating color intensity to the concentration of the Free Testosterone.
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- Delivery Time: Immediate
- Packaging Details: 1 Kit
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Calbiotech Beta2 Microglobulin Elisa Kit
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Product Details:
| Number of Reactions(Preps)/Kit | 96 Wells |
| Brand | Calbiotech |
| Product Type | ELISA Kit |
| Packaging Type | Box |
| Usage/Application | Hospital / Pathlab |
| Storage and Stability | Product should be stored at 2-8 DegreeC |
The B2MG ELISA test is based on the principle of a solid phase enzyme-linked immunosorbent assay. The assay system utilizes a unique monoclonal antibody directed against a distinct antigenic determinant on the intact 2 Microglobulin molecule. Mouse monoclonal anti- B2MG antibody is used for solid phase immobilization (on the microtiter wells). A sheep anti-B2MG antibody is in the antibody-enzyme (horseradish peroxidase) conjugate solution. The diluted test sample is allowed to react first with the immobilized antibody for 30 minutes at 37 C. The sheep antiB2MG-HRP conjugate is then added and reacted with the immobilized antigen for 30 minutes at 37 C, resulting in the B2MG molecules being sandwiched between the solid phase and enzyme-linked antibodies. The wells are washed with water to remove unbound-labeled antibodies. A solution of TMB Reagent is added and incubated for 20 minutes at room temperature, resulting in the development of a blue color. The color development is stopped with the addition of Stop Solution, changing the color to yellow. The concentration of B2MG is directly proportional to the color intensity of the test sample. Absorbance is measured spectrophotometrically at 450 nm.
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- Delivery Time: Immediate
- Packaging Details: 1 Kit
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Calbiotech CEA Elisa Kit
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Product Details:
| Minimum Order Quantity | 1 Kit |
| Number of Reactions(Preps)/Kit | 96 Wells |
| Brand | Calbiotech |
| Product Type | ELISA Kit |
| Packaging Type | Box |
| Usage/Application | Hospital / Pathlab |
| Storage and Stability | Product should be stored at 2-8 DegreeC |
The CBI CEA is a solid phase sandwich ELISA method. The samples, and anti-CEA-HRP/Biotin conjugate are added to the wells coated with Streptavidin. CEA in the patient’s sample forms a sandwich between two specific antibodies to CEA. Unbound protein and HRP conjugate are washed off by wash buffer. Upon the addition of the substrate, the intensity of color is proportional to the concentration of CEA in the samples. A standard curve is prepared relating color intensity to the concentration of the CEA.
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- Delivery Time: Immediate
- Packaging Details: 1 Kit
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Calbiotech Triiodothyronine (T3) Elisa Test
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Product Details:
| Number of Reactions(Preps)/Kit | 96 Wells |
| Brand | Calbiotech |
| Product Type | ELISA |
| Packaging Type | Box |
| Usage/Application | Hospital / Pathlab |
| Species | Human |
The T3 is a solid phases competitive ELISA. The samples, assay buffer and T3 enzyme conjugate are added to the wells coated with anti-T3 monoclonal antibody. T3 in the patient’s serum competes with a T3 enzyme conjugate for binding sites. Unbound T3 and T3 enzyme conjugate is washed off by washing buffer. Upon the addition of the substrate, the intensity of color is inversely proportional to the concentration of T3 in the samples. A standard curve is prepared relating color intensity to the concentration of the T3.
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- Packaging Details: 1 Kit
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Calbiotech FT3 Elisa Kit - Triiodothyronine, Free
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Product Details:
| Brand | Calbiotech |
| Usage/Application | Hospital / Pathlab |
| Pack Size | 96 Wells |
| Packing Type | Box |
| Category | Elisa |
| Species | Human |
The CBI fT3 is a solid phase competitive ELISA. The samples, and fT3 enzyme conjugate are added to the wells coated with anti-T3 monoclonal antibody. fT3 in the patient’s serum competes with T3 enzyme conjugate for binding sites in anti-T3 antibody. Unbound T3 and T3 enzyme conjugate is washed off by washing buffer. Upon the addition of the substrate, the intensity of color is inversely proportional to the concentration of fT3 in the samples. A standard curve is prepared relating color intensity to the concentration of the fT3.
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- Delivery Time: Immediate
- Packaging Details: 1 Kit
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Calbiotech TSH Elisa Kit
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Product Details:
| Minimum Order Quantity | 1 Kit |
| Number of Reactions(Preps)/Kit | 96 Wells |
| Brand | Calbiotech |
| Product Type | ELISA |
| Packaging Type | Box |
| Usage/Application | Hospital / Pathlab |
| Species | Human |
The CBI TSH is a solid phase sandwich ELISA method. The samples and anti-TSHHRP/Biotin conjugate are added to the wells coated with Streptavidin. TSH in the patient’s sample forms a sandwich between two specific antibodies to TSH. Unbound protein and HRP conjugate are washed off by wash buffer. Upon the addition of the substrate, the intensity of color is proportional to the concentration of TSH in the samples. A standard curve is prepared relating color intensity to the concentration of the TSH.
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- Delivery Time: Immediate
- Packaging Details: 1 Kit
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Calbiotech Hcg Elisa Kit
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Product Details:
| Minimum Order Quantity | 1 Kit |
| Number of Reactions(Preps)/Kit | 96 Wells |
| Brand | Calbiotech |
| Product Type | ELISA Kit |
| Packaging Type | Box |
| Category | Elisa |
| Usage/Application | Hospital / Pathlab |
| Species | Human |
The CBI hCG ELISA is an adapted solid phase sandwich ELISA method. The samples, biotin labeled antihCG and anti-hCG-HRP conjugate are added to the wells coated with Streptavidin. hCG in the patient’s serum binds to binds to the anti-hCG antibodies and forms a sandwich on the streptavidin coated plate. Unbound protein and HRP conjugate are washed off by wash buffer. Upon the addition of the substrate, the intensity of color is proportional to the concentration of hCG in the samples. A standard curve is prepared relating color intensity to the concentration of hCG in the sample
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- Delivery Time: Immediate
- Packaging Details: 1 Kit
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Calbiotech LH Elisa Kit
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Product Details:
| Number of Reactions(Preps)/Kit | 96 Wells |
| Brand | Calbiotech |
| Product Type | ELISA |
| Packaging Type | Box |
| Category | Elisa |
| Usage/Application | Hospital / Pathlab |
| Species | Human |
The LH ELISA kit is a solid phase assay using streptavidin/biotin method. The samples and Anti-LH/AntiBiotin conjugate are added to the wells coated with Streptavidin. LH in the patient’s serum forms a sandwich between specific antibodies labeled with biotin and HRP. Unbound protein and HRP conjugate are washed off by wash buffer. Upon the addition of the substrate, the intensity of color is proportional to the concentration of LH in the samples. A standard curve is prepared relating color intensity to the concentration of the LH.
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- Delivery Time: Immediate
- Packaging Details: 1 Kit
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Calbiotech Cortisol Elisa Kit
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Product Details:
| Number of Reactions(Preps)/Kit | 96 Wells |
| Brand | Calbiotech |
| Product Type | ELISA |
| Packaging Type | Box |
| Category | Elisa |
| Usage/Application | Hospital / Pathlab |
| Species | Human |
The Calbiotech, Inc. Cortisol test kit is a solid phase competitive ELISA. The samples, working Cortisol-HRP Conjugate and anti-cortisol-biotin solution are added to the wells coated with streptavidin. Cortisol in the patient’s serum competes with the cortisol enzyme (HRP) conjugate for binding sites. Unbound cortisol and cortisol enzyme conjugate is washed off by washing buffer. Upon the addition of the substrate, the intensity of color is inversely proportional to the concentration of Cortisol in the samples. A standard curve is prepared relating color intensity to the concentration of the cortisol.
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- Delivery Time: Immediate
- Packaging Details: 1 Kit
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Calbiotech Ferritin Elisa Kit
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Product Details:
| Minimum Order Quantity | 1 Kit |
| Number of Reactions(Preps)/Kit | 96 Wells |
| Brand | Calbiotech |
| Product Type | ELISA |
| Packaging Type | Box |
| Category | Elisa |
| Usage/Application | Hospital / Pathlab |
| Species | Human |
This assay will recognise both natural and recombinant human Ferritin
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- Delivery Time: Immediate
- Packaging Details: 1 Kit
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Calbiotech Cardiolipin Total Ab Elisa Kit
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Product Details:
| Number of Reactions(Preps)/Kit | 96 Wells |
| Brand | Calbiotech |
| ICMR Approved | Yes |
| Product Type | ELISA |
| Packaging Type | Box |
| Usage/Application | Hospital / Pathlab |
Diluted patient serum is added to wells coated with purified aCL antigen. aCL specific antibody, if present, binds to the antigen. All unbound materials are washed away and the enzyme conjugate is added to bind to the antibody-antigen complex, if present. Excess enzyme conjugate is washed off and substrate is added. The plate is incubated to allow the hydrolysis of the substrate by the enzyme. The intensity of the color generated is proportional to the amount of specific antibody in the sample.
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- Packaging Details: 1 Kit
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Calbiotech Estradiol Elisa Kit
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Minimum Order Quantity: 1 Kit
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Product Details:
| Number of Reactions(Preps)/Kit | 96 Wells |
| Brand | Calbiotech |
| Packaging Type | Box |
| Category | Elisa |
| Usage/Application | Hospital / Pathlab |
| Species | Human |
The Calbiotech, Inc E2 ELISA kit is based on the principle of Delayed competitive binding assay between E2 in the test specimen and E2 enzyme conjugate for a constant amount of anti-Estradiol monoclonal antibody epitops (Biotin reagent). In the incubation, anti-E2 antibody biotin reagent, E2 standards, controls, and samples are incubated for 45 minutes at room temperature (RT), then E2 enzyme conjugate is added on the top of the reaction mixture and incubation continues for 45 minutes more. During the incubation, a fixed amount of HRP-labeled E2 competes with the endogenous E2 in the standard, sample, or quality control serum for a fixed number of binding sites of the specific E2 antibody. E2 peroxidase conjugate immunologically bound to the well progressively decreases as the concentration of E2 in the specimen increases. Unbound of anti-Estradiol Biotin Reagent and E2 peroxidase conjugate is then removed and the wells are washed. Next, a solution of TMB Reagent is added and incubated at room temperature for 20 minutes, resulting in the development of blue color. The color development is stopped with the addition of stop solution, and the absorbance is measured spectrophotometrically at 450 nm. A standard curve is obtained by plotting the concentration of the standard versus the absorbance
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- Packaging Details: 1 Kit
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Calbiotech Insulin Elisa Kit
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Minimum Order Quantity: 1 Kit
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Product Details:
| Number of Reactions(Preps)/Kit | 96 Wells |
| Brand | Calbiotech |
| Product Type | ELISA |
| Packaging Type | Box |
| Usage/Application | Hospital / Pathlab |
| Category | Elisa |
The Calbiotech Inc., Insulin ELISA is based on solid phase sandwich ELISA method. The samples and conjugate reagent (anti-Insulin biotin & HRP) are added to the wells coated with Streptavidin. Insulin in the patient's serum binds to the matched pair Abs, forming a sandwich complex and simultaneously the complex is being immobilized on the plate through streptavidin-biotin interactions. Unbound protein and HRP conjugate are washed off, through a washing step. Upon addition of the substrate, the intensity of color is proportional to the concentration of Insulin in the samples. A standard curve is prepared by relating the color intensity to the concentration of Insulin.
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- Delivery Time: Immediate
- Packaging Details: 1 Kit
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Calbiotech Growth Hormone Elisa Kit
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Minimum Order Quantity: 1 Kit
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Product Details:
| Number of Reactions(Preps)/Kit | 96 Wells |
| Brand | Calbiotech |
| Product Type | ELISA Kit |
| Packaging Type | Box |
| Usage/Application | Hospital / Pathlab |
| Species | Human |
1. Store the kit at 2 - 8°C.
2. Keep microwells sealed in a dry bag with desiccants.
3. The reagents are stable until expiration of the kit.
4. Do not expose reagent to heat, sun, or strong light.
REAGENTS PREPARATION -
Prepare 1X Wash buffer by adding the contents of the bottle (25 ml, 20X) to 475 ml of distilled or deionized water. Store at room temperature (20-25°C).
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- Packaging Details: 1 Kit
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Calbiotech C-Reactive Protein Elisa Kit
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Minimum Order Quantity: 1 Kit
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Product Details:
| Number of Reactions(Preps)/Kit | 96 Wells |
| Brand | Calbiotech |
| Product Type | ELISA Kit |
| Packaging Type | Box |
| Usage/Application | Hospital / Pathlab |
| Storage and Stability | Product should be stored at 2-8 DegreeC |
1. Store the kit at 2 - 8°C.
2. Keep microwells sealed in a dry bag with desiccants.
3. The reagents are stable until expiration of the kit.
4. Do not expose test reagents to heat, sun, or strong light.
REAGENTS PREPARATION -
1X Wash Buffer: Prepare 1X Wash buffer by adding the contents of the bottle (25 ml, 20X) to 475 ml of distilled or deionized water. Store at room temperature (20-25°C).
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- Packaging Details: 1 Kit
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Calbiotech AFP Elisa Kit
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Minimum Order Quantity: 1 Kit
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Product Details:
| Number of Reactions(Preps)/Kit | 96 Wells |
| Brand | Calbiotech |
| Product Type | ELISA Kit |
| Packaging Type | Box |
| Usage/Application | Hospital / Pathlab |
| Storage and Stability | Store the kit at 2 - 8 DegreeC |
This AFP ELISA kit is a solid phase sandwich assay method, based on a streptavidin-biotin principle. The standards, samples and the biotinylated Anti-AFP antibody reagent are added into designated wells, coated with Streptavidin. Endogenous AFP in the patient’s serum binds to the antigenic site of the biotinylated Anti-AFP antibody. Simultaneously, the biotinylated antibody is immobilized onto the wells through the high affinity Streptavidin-Biotin interaction. Unbound protein and excess biotin conjugated antibody are washed off by wash buffer. Upon the addition of the Peroxidase (HRP) conjugated Anti-AFP antibody reagent, a sandwich complex is formed, the analyte of interest being in between the two highly specific antibodies, labeled with Biotin and HRP. Unbound protein excess enzyme conjugated antibody reagent is washed off by wash buffer. Upon the addition of the substrate, the intensity of color developed is directly proportional to the concentration of AFP in the samples. A standard curve is prepared relating color intensity to the concentration of the AFP.
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- Delivery Time: Immediate
- Packaging Details: 1 Kit
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Calbiotech CA-125 Elisa Kit
Product Price: Rs 1 / KitGet Best Price
Minimum Order Quantity: 1 Kit
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Product Details:
| Number of Reactions(Preps)/Kit | 96 Wells |
| Brand | Calbiotech |
| Product Type | ELISA Kit |
| Packaging Type | Box |
| Usage/Application | Hospital / Pathlab |
| Storage and Stability | Product should be stored at 2-8 DegreeC |
The CA125 ELISA test is an adapted solid phase 1-step sandwich ELISA. Samples, a biotinylated mouse anti-CA-125 capture antibody, and mouse anti-CA-125-HRP conjugate are all added to wells coated with streptavidin. CA-125 in the patient sample binds to the biotinylated capture antibody. The biotinylated capture antibody simultaneously binds to the streptavidin coated plate. Anti-CA-125–HRP enzyme conjugate forms a sandwich around captured CA-125. Unbound antibodies are washed off. TMB substrate is added resulting in the development of a blue color. The concentration of CA-125 is directly proportional to the color intensity developed. A standard curve is generated relating color intensity to CA-125 concentration.
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- Delivery Time: Immediate
- Packaging Details: 1 Kit
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Calbiotech ANA Screen IgG Elisa Kit
Product Price: Rs 1 / KitGet Best Price
Minimum Order Quantity: 1 Kit
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Product Details:
| Number of Reactions(Preps)/Kit | 96 Wells |
| Brand | Calbiotech |
| ICMR Approved | Yes |
| Product Type | ELISA Kit |
| Packaging Type | Box |
| Usage/Application | Hospital / Pathlab |
Diluted patient serum is added to wells coated with purified nuclear antigens. ANA IgG specific antibody, if present, binds to the antigen. All unbound materials are washed away and the enzyme conjugate is added to bind to the antibody-antigen complex, if present. Excess enzyme conjugate is washed off and substrate is added. The plate is incubated to allow the hydrolysis of the substrate by the enzyme. The intensity of the color generated is proportional to the amount of IgG specific antibody in the sample.
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- Delivery Time: Immediate
- Packaging Details: 1 Kit
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Calbiotech Cardiolipin IgA Elisa Kit
Product Price: Rs 1 / KitGet Best Price
Minimum Order Quantity: 1 Kit
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Product Details:
| Number of Reactions(Preps)/Kit | 96 Wells |
| Brand | Calbiotech |
| ICMR Approved | Yes |
| Product Type | ELISA |
| Packaging Type | Box |
| Usage/Application | Hospital / Pathlab |
Diluted patient serum is added to wells coated with purified aCL antigen. aCL specific IgA antibody, if present, binds to the antigen. All unbound materials are washed away and the enzyme conjugate is added to bind to the antibody-antigen complex, if present. Excess enzyme conjugate is washed off and substrate is added. The plate is incubated to allow the hydrolysis of the substrate by the enzyme. The intensity of the color generated is proportional to the amount of specific antibody in the sample.
Additional Information:
- Delivery Time: Immediate
- Packaging Details: 1 Kit
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Calbiotech Cardiolipin IgM Elisa Kit
Product Price: Rs 1 / KitGet Best Price
Minimum Order Quantity: 1 Kit
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Product Details:
| Number of Reactions(Preps)/Kit | 96 Wells |
| Brand | Calbiotech |
| ICMR Approved | Yes |
| Product Type | ELISA |
| Packaging Type | Box |
| Usage/Application | Hospital / Pathlab |
Diluted patient serum (serum diluent contains sorbent to remove Rheumatoid Factor and human IgG interference) is added to wells coated with purified aCL antigen. aCL specific IgM antibody, if present, binds to the antigen. All unbound materials are washed away and the enzyme conjugate is added to bind to the antibody-antigen complex, if present. Excess enzyme conjugate is washed off and substrate is added. The plate is incubated to allow the hydrolysis of the substrate by the enzyme. The intensity of the color generated is proportional to the amount of specific IgM antibody in the sample.
Additional Information:
- Delivery Time: Immediate
- Packaging Details: 1 Kit
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Calbiotech dsDNA IgG Elisa Kit
Product Price: Rs 1 / KitGet Best Price
Minimum Order Quantity: 1 Kit
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Product Details:
| Number of Reactions(Preps)/Kit | 96 Wells |
| Brand | Calbiotech |
| ICMR Approved | Yes |
| Product Type | ELISA Kit |
| Packaging Type | Box |
| Usage/Application | Hospital / Pathlab |
Diluted patient serum is added to wells coated with purified dsDNA antigen. Specific antibody, if present, binds to the antigen. All unbound materials are washed away and the enzyme conjugate is added to bind to the antibody-antigen complex, if present. Excess enzyme conjugate is washed off and substrate is added. The plate is incubated to allow the oxidation of the substrate by the enzyme. The intensity of the color generated is proportional to the amount of specific antibody in the sample.
Additional Information:
- Delivery Time: Immediate
- Packaging Details: 1 Kit
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Calbiotech Torch Panel Test Kit
Product Price: Rs 1 / KitGet Best Price
Minimum Order Quantity: 1 Kit
Product Details:
| Number of Reactions(Preps)/Kit | 96 Wells |
| Brand | Calbiotech |
| Product Type | ELISA |
| Packaging Type | Box |
| Usage/Application | Hospital / Pathlab |
| Category | Elisa |
[ CMV IgG, CMV IgM, HSV1 IgM, HSV2 IgM, HSV1&2 IgM, HSV1 IgG, HSV2 IgG, HSV1&2 IgG, Rub IgG, Rub IgM, Toxo IgG, Toxo IgM ]
Additional Information:
- Delivery Time: Immediate
- Packaging Details: 1 Kit
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Calbiotech Cardiolipin IgG Elisa Kit
Product Price: Rs 1 / KitGet Best Price
Minimum Order Quantity: 1 Kit
Product Details:
| Number of Reactions(Preps)/Kit | 96 Wells |
| Brand | Calbiotech |
| ICMR Approved | Yes |
| Product Type | ELISA |
| Packaging Type | Box |
| Usage/Application | Hospital / Pathlab |
Diluted patient serum is added to wells coated with purified aCL antigen. aCL specific IgG antibody, if present, binds to the antigen. All unbound materials are washed away and the enzyme conjugate is added to bind to the antibodyantigen complex, if present. Excess enzyme conjugate is washed off and substrate is added. The plate is incubated to allow the hydrolysis of the substrate by the enzyme. The intensity of the color generated is proportional to the amount of specific antibody in the sample.
Additional Information:
- Delivery Time: Immediate
- Packaging Details: 1 Kit
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